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SYMPHONY automated peptide synthesiser

The BRF synthesises high quality peptides using a state-of-the-art SYMPHONY/MULTIPLEX multiple peptide synthesiser. This instrument is capable of simultaneously producing peptides. The core of the instrument is a multiplexing matrix valve that permits different reagents to be transferred simultaneously to different reaction vessels without cross contamination. Different reaction times and protocols can be applied for each synthesis (or even each amino acid). Synthesis in any reaction vessel can start or stop at any time regardless of whether syntheses are being carried out in other reaction vessels. Reagents are dispersed from common resevoirs for the synthesis of all peptides. The ACRF BRF is capable of producing 12 peptides per month.

Syntheses are performed using FMOC chemistry and solid phase peptide synthesis (SPSS) techniques. Peptides are synthesised at the 80 mmol scale with peptide production time taking between 1-3 weeks depending on length of peptide and whether purification is required. We routinely synthesise peptides up to 30 amino acids but are capable of synthesising longer peptides and are willing to attempt more difficult syntheses. The Facility is also able to provide chemically-modified peptides. Modifications include acetylation, N-terminal labelling (eg. biotin and fluorescein) and conjugation (eg. to KLH or MAPS). We can also synthesise peptides incorporating D-amino acids or unusual amino acids. Please contact us to discuss your peptide synthesis requirements.

The standard scale of synthesis is 80 mmole. The yield of product depends on the peptide sequence and level of purity required. No guarantee can therefore be given for the final yield. Peptides are purified via reverse-phase HPLC and the quality checked by mass spectroscopy.

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Ordering
Online via your web browser using peptideLIMS The number of peptides in the queue can be viewed by accessing the "View or Delete a Synthesis Request" link in peptideLIMS user tools.

Pricing

Contact
Kerry McAndrew
Tel: 6125 4326
email: Kerry.Mcandrew@anu.edu.au